Abstract. Streptomyces play a role in the degradation process of organic compounds that exist on the soil, especially in the mangrove area through several enzyme activities. These bacteria can help in providing organic ingredients as a food source for organisms that live around mangroves including the nipah worms (Namalycastis rhodochorde). This study aims to determine the enzyme activity profile of seven actinomycetes isolates that have been isolated from mangrove soil in Sungai Kakap district, West Kalimantan. Determining of enzymatic activity was carried out in asparagine glycerol agar which was added with starch, milk casein, cellulose and gelatine. The results showed that seven Streptomyces isolates had proteolytic, cellulolytic, amylolytic, and gelatin degradation activities. Streptomyces sp. NrASA1 has the highest proteolytic activity index values, namely 0.9 and Streptomyces sp. NrASA3 has the highest amylolytic and cellulolytic activity index values, respectively 0.8 and 0.7.