Polychaete Nereis sp. belongs to Nereidae family that lives as benthic organism in estuarine. The high level of amino acid and unsaturated fatty acid in Nereis sp. is potentially used as nutrient in prawn feed. These compositions increase the quality of prawn gametes and larva. However, there is still less information about the nutrient composition of Nereis sp. in Jeruklegi area. Therefore, a study about nutrient composition (chemical composition) of Nereis sp. in this area was conducted as the basic information for the Nereis sp. culture in the future. This study used survey method and sampling were conducted in four stations with three replications were allocated to each three-sampling point in each station every two week. The result showed no significant differences among the protein and fat composition of Nereis sp. from each station. The range 42,06 – 51,68% of protein and 12,93 – 22% of fat can fulfil the requirements in crustacean farming. The organic materials in this coastal area was considerably high therefore it is potential for Nereis sp. culture.