Southeast Maluku is the main producer of Indonesian trepang, but lately the resources have been dropped significantly. The effort to multiply trepang through sexual reproductive techniques still requires a long time. Therefore, another method is needed that is faster, more effective, and easier to implement by coastal communities, namely with inducing asexual reproduction of fission using a rubber band. This research using experimental methods in the field and analyzed descriptively. Fission induction conducted by binding 1/3 of the body part anterior to each individual of trepang. This study induces asexual reproduction in two trepan species found on Matakus Island, Tanimbar Archipelago, Maluku, Holothuria scabra and Bohadschia marmorata. The results showed that H. scabra (98%) and B. marmorata (100%) can induced fission and divide into two part. The posterior part has a higher regeneration and a better survival rate than the anterior part. The survival rate of B. marmoratais higher than that of H. scabra. The method of inducing asexual reproduction with rubber band can be used by Maluku coastal communities as an added value for their local wisdom called “sasi”.