Winged bean (Psophocarpus tetragonolobus (L.) (DC.) contains moderate to high fat in its seeds including erucic acid (C22:1) which is important raw-material of various oleochemical industries. ?-ketoacyl-CoA synthase (KCS) is one among those enzyme involves in erucic acid biosynthesis pathway by condensating acyl-CoA to ?-ketoacyl-CoA. Information about encoding genes of the KCS enzyme involve in erucic acid biosynthesis pathway, therefore, becomes a prerequisite. Current data show the paired degenerated primers of forward primer 5’-ATNTTCAACCCGACDCCDTC-3’ and reverse 5’-GAGCTTCACCTCCAACATYS-3’. PCR resulted in amplicons of 404 bp and 301 bp which are predicted as putative fragments of the KCS enzyme encoding genes involves in the erucic acid biosynthesis in winged bean.