Preservation is a technique to protect microbes from damage and maintain their viability for further use in the future with minimum character changes. Base on the storage time, there are two techniques: short-term preservation that requires regrown of the microbes periodically into a new media, and long-term preservation such as cryopreservation and freeze-drying. The long-term preservation technique was proven to decrease the microbe metabolism rate and induce dormancy. This work aimed to examine the preservation techniques of filamentous fungi based on inactive metabolism, and to test the viability of cryopreservation technique. In this work, the filamentous fungi collection of Indonesian Culture Collection (InaCC) were kept in a deep freezer (-80°C) while added with a cryoprotectant (10%  glycerol and 5% trehalose). After being preserved for about three years, 80% of the fungi were viable. The remaining 20% showed a low rate of viability thus hinder the fungal growth. It was suggested that the cryopreservation technique of -80°C, 10% glycerol and 5% trehalose cryoprotectant was the most suitable technique for many fungal cultures.

Hasnadhiazahra Rohadi

Muhammad Ilyas

Nuraeni Ekowati