Several studies reported fish larvae diversity from East part of Segara Anakan. No such study has been reported from the lagoon and west part of that areas, either morphologically or molecular. Morphological identification of fish larvae faces difficulties which might lead to miss-identification. Molecular barcode are reliable characters for a proper species level identification. Here we used cytochrome c oxidase 1 gene as barcode marker for species level identification of fish larvae from the lagoon and west areas of Segara Anakan. The gene was sequenced using big dye terminator methods. Sequences homologous were checked to previously published sequences available in the GenBank and BOLDsystem. Taxonomic tree was created using neighbor-joining methods in MEGA6 software. Branching polarity was supported by 1 000 bootstraps pseudo-replicates.The Kimura 2-Parameter genetic divergences were calculated. A total of 1 257 larvae were collected during the field trips. Microscopic observation placed the samples into 21 morphotypes. Fourteen out of the 21 morphotypes were convincingly identified into species level due to their high homology with certain conspecific sequences in GenBank (97.87% to 100%), while one remaining morphotype could only be identified into generic level with rather lower homology (87%). Taxonomic tree shows clear grouping between samples and their conspecific individuals and shows clear separation among morphotypes. Genetic distances among morphotypes were ranges from 0.164 to 0.295. Similarity test, branch topology, and genetic distances proved that most of the larvae were precisely identified into species or genus levels depend on the availability of sequences homolog in the data bases.

Agus Nuryanto

Hendro Pramono

Moh. Husein Sastranegara

Kusbiyanto