There are two types of antioxidant compounds, endogenous and exogenous antioxidants. Endogenous antioxidants such as superoxide dismutase (SOD) are enzymatic antioxidants, and exogenous antioxidants such as flavonoids are chain reaction breakers. Uptake of Cu and Zn which functions as a cofactor influence synthesis of the enzyme. This study focused on flavonoid type of planting media and the effective concentration of humic acid in Cu and Zn uptake, in the Daun Dewa. The research applied the experimental method according to a Split Plot design with three replicates. Planting media (sand and zeolite) was the main plot, and concentrations of humic acid (0, 4, 8, and 12 g/kg) were the subplots. The parameters were Cu and Zn uptake, SOD activity, antioxidant activity test, and flavonoid content. The results showed that the planting media and the concentration of humic acid affected Cu and Zn uptake. Sand planting medium with 12 g/kg humic acid increased Cu uptake. Zn uptake improved in post-planting medium with 4 g/kg humic acid. SOD activity and flavonoid content increased in the sand medium of 4 g/kg humic acid. Zeolite planting media with 8 g/kg humic acid promoted exogenous antioxidant activity.

Elly Proklamasiningsih

Iman Budisantoso

Kamsinah

Pudji Widodo