Winged bean (Psophocarpus tetragonolobus (L.) DC) is an alternative food source that can be optimally cultivated. An application of mutation techniques on winged bean can provide genetic variation important for agricultural industry, especially in assembling the superior cultivars. This research aimed to determine anatomical response and chlorophyll content of mutated winged plants, and to find out the anatomical character differences between mutated and non-mutated winged leaves. The research was experiments based on a Completely Randomized Design (CRD) with a factorial pattern. Factor I was winged plants and factor II was the concentration of Cobalt 60 radiation (0, 25, 50, 75 and 100 gray), with five replicates. The results showed that the Cobalt 60 gamma ray radiation increased the cuticle and leaf mesophyll thickness, but decreased the length and stomatal density of the leaves. Cobalt 60 ray radiation had no effects on the leaf chlorophyll content.

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