The SEA+ Conference on Biodiversity and Biotechnology 2018

The Production of Pectinase Enzymes by Colletotrichum acutatum Simmonds. Causing Anthracnoses in Red Chilli (Capsicum annuum, L.)

Juni Safitri Muljowati (1) , Loekas Soesanto (2) , Laurensius Hartanto Nugroho (3)


(1) Faculty of Biology, Universitas Jenderal Soedirman
(2) Universitas Jenderal Soedirman
(3) Universitas Gadjah Mada

Building: Java Heritage Convention
Room: Room II
Date: 2018-11-06 09:45 – 10:00
Last modified: 2019-05-10

Abstract


Almost all pathogenic fungi produce pectinase enzymes to degrade the host cell wall during pathogenesis. The mode of action of C. acutatum is no different from other Colletotrichum species. Colletotrichum fungi produce cutinase and pectinase enzymes to weaken the host's defenses and help further infection. The occurrence of anthracnose disease was determined by the success of pathogenesis by C. acutatum. During pathogenesis, in addition to the enzyme cutinase, the enzyme that plays an important role is pectinase which is used to degrade the host cell wall. This paper discusses the ability of six C. acutatum isolates from Java to produce pectinase enzymes by which have been known to have the highest pathogenicity at each sampling location (Malang, Temanggung, Kulonprogo, Brebes, Garut, and Pandeglang). The results showed that all C. acutatum isolates could produce pectinase. There was a difference in the ability to macerate enzymes even though enzyme quality test results did not differ.


Juni Safitri Muljowati


Keywords


pectinase enzyme, Colletotrichum acutatum, Antraknosa, Capsicum annuum