There are several interaction patterns of fungus that live in the rhizosphere of plants, i.e. mutualism, commensalism, saprophytic, and parasitic. The objectives of this study was to determine the genera of microscopic fungus collected from the rhizosphere of groundnut, mays plant, and rice field. In addition, the biodiversity of soil fungi of those three plants was considered. Research method applied was survey with purposive random sampling. The rhizospheric soil sample was obtained from research locations of ground nut, mays plants, and rice field in Purwokerto area. The soil sample were taken from 1-10 cm deep, isolate them, and pure culture preparation as well. Furthermore, obtained data of fungus collection were then analysed descriptively, and also described based on their macro and micro morphology. Then, the collected fungus were identified by using identification manual for fungus.  Result showed that fifteen (15) isolates were found in three different rhizosphere of ground nut, mays plants, and rice field of Purwokerto. The description is as follow: Aspergillus-1, Aspergillus-2, Penicillium-1, Penicillium-2, Mycophyta, Aureobasidia, Cylindrocarpon, Mucor-1, Mucor-2, Chaetomium, Gliocladium, Trichoderma-1, Trichoderma-2, Pleurophragmium, and Gonytrichum.  Finally, the Biodiversity Index (H’) noted from rhizosphere of groundnut plants was 0.312; mays was 0.195; and (H’) of rice field was 0.124.

rhizosphere, macromorphology, micromorphology, identification, genera