Streptomyces sp. SA32 isolated from mangrove rhizosphere in Segara Anakan, Cilacap, Indonesia, showed antibacterial activity against Multi-Drug Resistance (MDR) bacteria. The inhibitory activity was shown by the MIC test with a concentration of 20%  ethyl acetate crude extract of Starch Casein Nitrate Broth (SCNB) medium. The study of antibacterial compounds by thin layer chromatography (TLC) demonstrated three different spots, but the class of compounds produced was not yet known. The research was conducted to improve the antibacterial ability of SCNB filtrate compounds with the addition of glucose and incubation period of 7, 14 and 21 days, and to determine the class of bioactive compounds with the phytochemical test method. The results showed that the addition of 1% glucose with an incubation period of 14 days exhibited the highest antibacterial activity, and isolate Streptomyces sp. SA32 produced polyketide and flavonoid compounds.

Dini Ryandini