The High Expression of MnSOD Gene in the Wound Healing Phase of Tail Tissue Regeneration of House Gecko (Hemidactylus platyurus)
(1) Doctoral Biomedical Program, Faculty of Medicine University of Indonesia; Biotechnology Department, Universitas Esa Unggul, Indonesia<br />
(2) Department of Pathology, Faculty of Animal Health, Agriculture Institute of Bogor, Indonesia
(3) Department of Histology, Faculty of Medicine, University of Indonesia
(4) Institute of Indonesia Science (LIPI), Cibinong, Bogor, Indonesia
(5) Center of Hypoxia, Biochemistry Department, Faculty of Medicine, University of Indonesia
(6) Center of Hypoxia, Biochemistry Department, Faculty of Medicine, University of Indonesia
Building: Java Heritage Convention
Room: Room II
Date: 2018-11-07 10:45 – 11:00
Last modified: 2019-02-12
Abstract
Humans are living beings who have limited ability in the tissue regeneration process. Research about tissue regeneration continues to develop the human tissue regeneration process. Various animal models are used to analyze tissue regeneration processes. Reptiles, especially house gecko (Hemidactylus platyurus), have a very high regeneration ability on their tails. House gecko can be used as animal models because they are easy to obtain, have the ability to autotomy, and regrow their tails. In the tissue regeneration process, the cells are active to proliferate, migrate, and differentiate. This high activity causes the tissue to require high oxygen and energy. The imbalance between oxygen in tissues and external oxygen supply causes the tissue in a hypoxic state. This condition can cause exposure of the free radical compounds including ROS (Reactive Oxygen Species) which can damage the cells. In the cell, there is an enzyme of superoxide dismutase (SOD) which can convert radical compounds into compounds that are safe for cells. The SOD enzyme compounds in the mitochondria are Manganese superoxide dismutase (MnSOD). This research used the 30 adult female of house geckos that had never experienced autotomy. After autotomy the tail that using procedures of Zoology Laboratory of Indonesian Science Institute. After the tail regenerated on days 1, 3, 5, 8, 10, 13, 17, 21, 25, and 30, the tissue was taken to be observed histologically and analyzed for mRNA expression. Expression of mRNA MnSOD using qRT-PCR showed increasing expression from day 5 and peaked at day 8. On day 8, nerve cell density, fibroblast cell density, epidermal thickness, and dermis thickness began to increase significantly. The results showed that MnSOD had a role in stimulating the cell to grow which played a role in tissue regeneration. The results of the correlation test between MnSOD expression and growth length of house gecko tails were negative moderate. It showed that after MnSOD stimulation of cell activity, the cell began to increase its activity for tissue regeneration.